Mathematical bioPhysics

The group pursues a mathematical physics-approach to study phenomena in biophysics.

Our main research focus is the non-equilibrium statistical mechanics of single molecules/particles and the collective behavior of larger molecular and up to sub-cellular assemblies. In particular, we aim at a trajectory-based description of macromolecular conformation dynamics as well as of their spatial transport, binding, and reactions. From a fundamental perspective we are focusing on relaxation phenomena of Markovian and non-Markovian observables from far-from-equilibrium quenches as well as the generic physical origin and understanding of broken time-translation invariance. In the analysis of stochastic many-body systems we aim at an understanding of emerging collective effects from a trajectory perspective.  In our work we employ rigorous analysis corroborated by computer simulations. Please see Recent research activities below for more details.


Recent research highlights

Heating and cooling are fundamentally asymmetric

Using an optically trapped colloidal particle, we show, in collaboration with the group of Raul Rica (Univ. de Granada)  that microscale systems quenched far from thermodynamic equilibrium heat up faster than they cool down. We show, by experiment and by mathematical proofs, that between any pair of temperatures, heating is not only faster than cooling but the respective processes in fact evolve along fundamentally distinct pathways, which we explain with a new theoretical framework that we coin “thermal kinematics”. Our results change the view of thermalization at the microscale are expected to have a strong impact on energy-conversion applications and thermal management of microscopic devices such as Brownian heat engines.

Ibáñez, M.; Dieball, C.; Lasanta, A.; Godec, A.; Rica, R. A.: Heating and cooling are fundamentally asymmetric and evolve along distinct pathways. Nature Physics (2024)

 

Some certainty about the uncertainty of kinetic inference

A notorious problem in inference is the uncertainty of estimates in the presence of limited or insufficient sampling. In the worst case scenario, large uncertainties are known to lead to erroneous conclusions. What is worse, so far it was not possible to make any reliable (unbiased) statements about the uncertainty of the estimates without substantial knowledge or intuition of the observed process, which is typically not available. By developing a new mathematical framework to control uncertainty of inferred rates and even their expected range of values in arbitrarily small samples, we provide certainty about the uncertainty of kinetic inference without any prior knowledge about the underlying dynamics. This finally opens the door to rigorous control and unbiased estimation of errors in the presence of limited sampling.

Bebon, R.; Godec, A.: Controlling Uncertainty of Empirical First-Passage Times in the Small-Sample Regime. Physical Review Letters 131 (23), 237101 (2023)

 

Direct route to thermodynamic uncertainty relations and their saturations

Thermodynamic uncertainty relations (TURs) bound the dissipation in nonequilibrium systems from below by fluctuations of any observed current. TURs have been developed in various flavors, depending on the physical setting. In contrast to elaborate techniques employed in derivations of respective TURs, we prove these TURs directly from the Langevin equation using stochastic calculus. This establishes the TUR as an inherent property of stochastic equations of motion, akin to quantum-mechanical uncertainty.  By including correlations, we further derive a new, sharpest TUR for transients. Our simplest and most direct proof unifies TURs and determines conditions under which they saturate, thus enabling a more accurate thermodynamic inference.

Dieball, C.; Godec, A.: Direct Route to Thermodynamic Uncertainty Relations and Their Saturation. Physical Review Letters 130 (8), 087101 (2023)

 

Thermodynamically consistent phase-field theory including nearest-neighbor pair correlations

Our current understanding of interracial phenomena and phase separation is mostly still based on ideas that disregard correlations between particles. In our work we illuminate unexpected effects of correlations on the structure and thermodynamics of interfaces and in turn phase separation, which are decisive in systems with strong interactions. We directly evaluate the continuum limit of the Ising model on the Bethe-Guggenheim level to derive a Cahn-Hilliard free energy that takes into account pair correlations. For a one-dimensional interface in a strip geometry, these give rise to an effective interface broadening at interaction strengths near and above the thermal energy, which is verified in the full Ising model. Interface broadening is the result of an entropy-driven interface delocalization, which is not accounted for in the widely adopted mean field theory. Pair correlations are required for thermodynamic consistency as they enforce a thermodynamically optimal local configuration of defects and profoundly affect nucleation and spinodal decomposition at strong coupling.

Blom, K.; Ziethen, N.; Zwicker, D.; Godec, A.: Thermodynamically consistent phase-field theory including nearest-neighbor pair correlations. Physical Review Research 5 (1), 013135 (2023)

 

Threefold necessity for coarse graining in time-average statistical mechanics

It is well known that all experiments interrogating individual molecules and particles are limited by a finite precision and finite statistics. Yet, existing theoretical approaches for estimating the probability density of a particle’s location and current from individual trajectories failed to account for that. Within the newly developed theoretical framework we explain why accounting for a finite resolution and statistical sampling is not only necessary for well defined mathematical and statistical properties, but is also beneficial for the inference of thermodynamic and kinetic properties in small systems arbitrarily far from thermodynamic equilibrium.

The work was published in a joint publication in Physical Review Letters and Physical Review Research.

Dieball, C.; Godec, A.: Coarse graining empirical densities and currents in continuous-space steady states. Physical Review Research 4, 033243 (2022)
Dieball, C.; Godec, A.: Mathematical, thermodynamical, and experimental necessity for coarse graining empirical densities and currents in continuous space. Physical Review Letters 129 (14), 140601 (2022)

 

Scattering fingerprints of two-state dynamics

 

Particle transport in complex environments such as the interior of living cells is often non-Fickian or anomalous, that is, it deviates from the laws of Brownian motion. Such anomalies may be the result of small-scale spatio-temporal heterogeneities in, or viscoelastic properties of, the medium, molecular crowding, etc. Often the observed dynamics displays multi-state characteristics—distinct modes of transport dynamically interconverting between each other in a stochastic manner. Reliably distinguishing between single- and multi-state dynamics is challenging and requires a combination of distinct approaches. To complement the existing methods relying on the analysis of the particle’s mean squared displacement, position- or displacement-autocorrelation function, and propagators, we developed a theoretical framework for two-state scattering signatures—the intermediate scattering function and dynamic structure factor—and applied it to the analysis of simple model systems as well as particle-tracking experiments in living cells. We considered inert tracer-particle motion as well as systems with an internal structure and dynamics. Our results may generally be relevant for the interpretation of state-of-the-art differential dynamic microscopy experiments on complex particulate systems, as well as inelastic or quasielastic neutron (incl. spin-echo) and x-ray scattering probing structural and dynamical properties of macromolecules, when the underlying dynamics displays two-state transport.

Dieball, C.; Krapf, D.; Weiss, M.; Godec, A.: Scattering fingerprints of two-state dynamics. New Journal of Physics 24, 023004 (2022)

 

Colloids dancing on a fractal carpet made of light

Particle dynamics frequently occur in complex environments; in technology, e.g. atoms moving on structured surfaces, as well as in nature, e.g. transcription factors moving in the cell’s nucleus. These dynamics have often been modeled assuming that the medium resembles a fractal. Fractals are idealized geometrical objects that appear identical on all length scales (see Figure). Whether fractal concepts can actually be applied, has been scrutinized in the present study. In collaboration with the group of Stefan Egelhaaf (Univ. of Düsseldorf) we used a model system consisting of colloidal particles moving in a laser speckle pattern to tie experimental observations reliably to a fractal structure whose fractal dimension we were able to tune. The agreement between experimental observations and theoretical predictions reveals that the particles explore the speckle in a self-similar, fractal manner at least over four decades in time and on length scales up to 20 times the particle radius. Our work establishes the requirements that are necessary for fractal diffusion to be applicable, and provides methods to extract the fractal dimension. The importance of a fractal medium is discussed in the context of gene regulation.

Zunke, C.; Bewerunge, J.; Platten, F.; Egelhaaf , S. U.; Godec, A.: First-passage statistics of colloids on fractals: Theory and experimental realization. Science Advances 8 (3), eabk0627 (2022)

 


 

Emergent memory and kinetic hysteresis in strongly driven networks

In elastic materials, friction is known to cause a phenomenon called hysteresis – more energy is required to stretch a rubber band than is released during unloading. As a result, the rubber band becomes warm. On microscopic scales where thermal fluctuations are important, such as in molecular motors operating far from equilibrium, hysteresis emerges in the form of a broken time-reversal symmetry. In our paper we report on a novel form of hysteresis that is of a purely kinetic nature and emerges as soon as a system does not locally equilibrate in metastable states. This gives rise to memory in the observed dynamics; that is, state changes depend on past states.

Kinetic hysteresis arises from the finite duration of transition paths, which are completed transitions between pairs of metastable states. We derive, for the first time, a network theory that preserves the microscopic dissipation and accounts for the finite duration of transition paths. We unravel three ingrained symmetries of the dynamics that determine the characteristics of the observed memory. These symmetries reveal hidden, vital information about the dynamics even in the limit where the hysteresis may become negligibly small. We use the theory to explain the counterintuitive “catch-bond” phenomenon, where a larger mechanical load prolongs the lifetime of an adhesion bond.

Our results pave the way toward a deeper understanding of violations of time-reversal symmetry in the presence of memory. In practice, the symmetries unraveled in our work allow for robustly detecting the coexistence of multiple transition pathways in a measured time series without resolving them individually.

Hartich, D.; Godec, A.: Emergent memory and kinetic hysteresis in strongly driven networks. Physical Review X 11 (4), 041047 (2021)

Criticality in cell adhesion

Cells, the fundamental building blocks of living organisms, stick to other cells via a process called cell adhesion. For multicellular organisms cell adhesion plays a crucial role in the immune response, wound healing, and cancer development. Cell adhesion is mediated by so-called adhesion bonds. The breaking and formation of individual adhesion bonds is coupled via thermal fluctuations of the anchoring cell membrane. The last two decades of experiments and theory have shown that cell adhesion may in fact be accurately described by a model originally developed to describe ferromagnetism: the Ising model.

In our work we generalize the Ising model in order to explain how membrane fluctuations and external forces on the cell induce many-body effects, and how these in turn affect the equilibrium behavior and dynamical properties of cell adhesion. Strikingly, changes in the membrane rigidity can prolong or shorten the mean time a cell adheres to a stiff substrate, which in the thermodynamic limit of many adhesion bonds leads to the notion of a novel kind of dynamical critical point. The existence of a dynamical critical point is equivalently implied in the context of magnetization reversal times in ferromagnetic materials.

Blom, K.; Godec, A.: Criticality in cell adhesion. Physical Review X 11 (3), 031067 (2021)

Thermodynamic limits to anomalous diffusion

Thermal, Brownian fluctuations in physical systems were found to be bounded by a universal physical relation called the "thermodynamic uncertainty relation". Concurrently, experiments tracking individual particles or molecules frequently show fluctuations that deviate strongly from the Gaussian laws of Brownian motion – a phenomenon commonly referred to as "anomalous diffusion".

In our work we show, for the first time, that the two seemingly disjoint if not orthogonal fields of research – stochastic thermodynamics and anomalous diffusion – may be fruitfully combined to derive a new universal physical bound on the experimentally often unknown temporal extent of anomalous diffusion.

The paper was covered in a Viewpoint in Physics: https://physics.aps.org/articles/v14/116

Hartich, D.; Godec, A.: Thermodynamic uncertainty relation bounds the extent of anomalous diffusion. Physical Review Letters 127 (8), 080601 (2021)

 


Introducing: Time-average statistical mechanics

Many experiments on soft and biological matter probe individual trajectories. It is typically not feasible to repeat these experiments sufficiently many times in to apply the traditional concepts of (ensemble) statistical mechanics. It is, however, straightforward to analyze such data by means of time-averaging along individual realizations. However, a correct rationalization and interpretation of such  time-averaged results requires the framework of “time-average statistical mechanics”.

In our work we develop a spectral-theoretic approach to describe fluctuations of time-average observables evolving from general (incl. non-equilibrium) initial conditions, and consider both, reversible and irreversible (i.e. driven) dynamics.  Our results are directly applicable to a diverse range of phenomena underpinned by time-average observables in physical, chemical, biological systems, such as single-particle tracking and single-molecule spectroscopy, and may also find important applications in econophysics.

 

Lapolla, A.; Hartich, D.; Godec, A.: Spectral theory of fluctuations in time-average statistical mechanics of reversible and driven systems. Physical Review Research 2 (4), 043084 (2020)

 


An unforeseen asymmetry in relaxation to equilibrium: Nanoscale warming is faster than cooling

According to elementary physics cooling and warming rates should be identical if conditions are the same. A thermodynamic system generally evolves, or “relaxes,” to minimize its free energy, and if the total free energy difference between the initial and final conditions is the same in both cases, warming and cooling should be equally fast. As a result of a subtle imbalance in how the probability distribution of any system evolves under conditions of warming or cooling we find, however, that relaxation happens faster “uphill” than “downhill”.

We prove that near stable minima and for all quadratic energy landscapes it is a general phenomenon that also exists in a class of non-Markovian observables probed in single-molecule and particle-tracking experiments. The asymmetry is a general feature of reversible overdamped diffusive systems with smooth single-well potentials and occurs in multiwell landscapes when quenches disturb predominantly intrawell equilibria. Our findings may be relevant for the optimization of stochastic heat engines.

The paper was covered in Physics Focus: https://physics.aps.org/articles/v13/144

 

Lapolla, A.; Godec, A.: Faster uphill relaxation in thermodynamically equidistant temperature quenches. Physical Review Letters 125 (11), 110602 (2020)

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