Instruments and Software

 

Our equipment includes two transmission electron microscopes for routine work: a Zeiss EM 900 and a LEO 912AB Omega. For volume electron microscopy we use a Zeiss Crossbeam 540, which is a focused ion beam-scanning electron microscope (FIB-SEM). The ion beam is used in-situ to ablate material. With this method, a defined volume of a sample can be made accessible for the sequential acquisition of images and subsequent 3D reconstruction. In addition, the scanning electron microscope can be used for 2D imaging of large-area samples using the ATLAS mosaic function if the larger context of the tissue is relevant for the examination. If required, scanning electron microscope views of specimens such as insects, worms or similar can also be obtained.