Methods and Analysis

 

Based on the scientific question, we use immuno-electron microscopy on ultrathin cryosections according to the Tokuyasu method for morphological analysis of samples embedded in plastic or for localization of molecules within the cell or tissue context. For ultrastructural analysis, the samples are either chemically fixed and conventionally embedded or high-pressure frozen (HPF) and embedded in resin after freeze substitution. While conventional preparations are more suitable for quantitative comparisons of phenotypes, high-pressure frozen samples often reveal detailed ultrastructures that may be lost in conventional preparation methods due to chemical fixation and embedding. We are continuously developing and modifying sample preparation protocols such as microwave-assisted sample preparation for specialized applications.