Following textbook wisdom, the resolution of light microscopy is limited by diffraction to about half the wavelength of light, which is why conventional light microscopes fail to distinguish object details that are closer together than ~200 nanometers. Stefan Hell and his group have broken this century-old barrier by developing, since the early 1990's, novel fluorescence microscopes featuring diffraction-unlimited spatial resolution. Thus, they also laid the foundation of a new scientific field: superresolution fluorescence microscopy, also called fluorescence nanoscopy.

Stefan Hell's group is developing light microscopes with a spatial resolution down to a few nanometers, particularly, but not exclusively, for imaging cells and tissue of a living organism. Prominent methods include STED and RESOLFT microscopy as well as concepts based on stochastic single-molecule switching such as GSDIM microscopy. To surpass the diffraction barrier, all these methods utilize a reversible transition or switch of fluorescent labels between a bright and a dark state. In combination with 4Pi microscopy, which is another concept developed by this group that uses two opposing lenses, the resolution can be increased in all spatial dimensions down to the nanometer scale. Since these superresolution concepts fundamentally rely on transitions between molecular states, novel labels are required that can be optically prepared in at least two different states. Consequently, the group also pioneers the chemical synthesis and application of new labeling methods and techniques to improve the performance of the labels’ switching behavior to separate close-by molecules.

The novel superresolution methods are destined to become primary tools for imaging living biological samples ranging from cells and tissues to small animals, having the potential to transform the life sciences.

Press releases & research news

<span><span><span><span><span>Tracking molecular movements with nanometer precision using MINSTED</span></span></span></span></span>

Researchers led by Stefan Hell at the Max Planck Institute (MPI) for Multidisciplinary Sciences in Göttingen and the MPI for Medical Research in Heidelberg have succeeded in using MINSTED microscopy to visualize movements of the motor protein kinesin with spatio-temporal precision in the nanometer per millisecond range. Hell’s team has thus extended the MINSTED imaging method to precise tracking of molecular movements. more

Werner-von-Siemens-Ring 2022 for Stefan Hell

Stefan Hell is awarded the prize together with BioNTech scientists Uğur Şahin, Özlem Türeci, Christoph Huber, and Katalin Karikó. The physicist succeeded in observing living cells at the molecular level using the novel super-resolution microscopy, the foundation said about its decision to honor Hell. (in German) more

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MaxPlanckResearch SPEZIAL 09
Outsmarting Optical Boundaries 
Trying to controvert a seemingly incontrovertible law is a hard job. And Stefan Hell discovered just how hard when he attempted to thwart the resolution limit of optical microscopes. Initially, his ideas fell on deaf ears. Today, however, Stefan Hell is a Director at the Max Planck Institute for Biophysical Chemistry and since 2014, Nobel laureate.
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