Publications of Ashwin Chari

Chari, A.: Peptide bonds strike back. IUCrJ 12 (Part 3), 12 (2025)

Witkowska, J.; Giżyńska, M.; Karpowicz, P.; Sowik, D.; Trepczyk, K.; Henneberg, F.; Chari, A.; Giełdoń, A.; Pierzynowska, K.; Gaffke, L. et al.; Węgrzyn, G.; Jankowska, E.: Blm10-Based Compounds Add to the Knowledge of How Allosteric Modulators Influence Human 20S Proteasome. ACS Chemical Biology 20 (2), pp. 266 - 280 (2025)

Soh, W. T.; Roetschke, H. P.; Cormican, J. A.; Teo, B.F.; Chiam, N. C.; Raabe, M.; Pflanz, R.; Henneberg, F.; Becker, S.; Chari, A. et al.; Liu, H.; Urlaub, H.; Liepe, J.; Mishto, M.: Protein degradation by human 20S proteasomes elucidates the interplay between peptide hydrolysis and splicing. Nature Communications 15 (1), 1147 (2024)

Funk, L.-M.; Poschmann, G.; Rabe von Pappenheim, F.; Chari, A.; Stegmann, K. M.; Dickmanns, A.; Wensien, M.; Eulig, N.; Paknia, E.; Heyne, G. et al.; Penka, E.; Pearson, A. R.; Berndt, C.; Fritz, T.; Bazzi, S.; Uranga, J.; Mata, R. A.; Dobbelstein, M.; Hilgenfeld, R.; Curth, U.; Tittmann, K.: Multiple redox switches of the SARS-CoV-2 main protease in vitro provide opportunities for drug design. Nature Communications 15, 411 (2024)

Chari, A.; Stark, H.: Prospects and Limitations of High-Resolution Single-Particle Cryo-Electron Microscopy. Annual Review of Biophysics 52, pp. 391 - 411 (2023)

Donath, T.; Šišak Jung, D.; Burian, M.; Radicci, V.; Zambon, P.; Fitch, A. N.; Dejoie, C.; Zhang, B.; Ruat, M.; Hanfland, M. et al.; Kewish, C. M.; van Riessen, G. A.; Naumenko, D.; Amenitsch, H.; Bourenkov, G.; Bricogne, G.; Chari, A.; Schulze-Briese, C.: EIGER2 hybrid-photon-counting X-ray detectors for advanced synchrotron diffraction experiments. Journal of Synchrotron Radiation, pp. 723 - 738 (2023)

Singh, K.; Bunzel, G.; Graf, B.; Yip, K. M.; Neumann-Schaal, M.; Stark, H.; Chari, A.: Reconstruction of a fatty acid synthesis cycle from acyl carrier protein and cofactor structural snapshots. Cell 186 (23), e16, pp. 5054 - 5067 (2023)

Yi, S.-H.; Petrychenko, V.; Schliep, J. E.; Goyal, A.; Linden, A.; Chari, A.; Urlaub, H.; Stark, H.; Rodnina, M. V.; Adio, S. et al.; Fischer, N.: Conformational rearrangements upon start codon recognition in human 48S translation initiation complex. Nucleic Acids Research 50 (9), pp. 5282 - 5298 (2022)

Rindfleisch, S.; Krull, M.; Uranga, J.; Schmidt, T.; Rabe von Pappenheim, F.; Kirck, L. L.; Balouri, A.; Schneider, T.; Chari, A.; Kluger, R. et al.; Bourenkov, G.; Diederichsen, U.; Mata, R. A.; Tittmann, K.: Ground-state destabilization by electrostatic repulsion is not a driving force in orotidine-5′-monophosphate decarboxylase catalysis. Nature Catalysis 5, pp. 332 - 341 (2022)

Henneberg, F.; Chari, A.: Chromatography-free purification strategies for large biological macromolecular complexes involving fractionated PEG precipitation and density gradients. Life 11, 1289 (2021)

Henneberg, F.; Chari, A.: Chromatography-free purification strategies for large biological macromolecular complexes involving fractionated PEG precipitation and density gradients. Life 11 (12), 1289 (2021)

Yip, K. M.; Fischer, N.; Paknia, E.; Chari, A.; Stark, H.: Atomic-resolution protein structure determination by cryo-EM. Nature 587 (7832), pp. 157 - 161 (2020)

Ukmar-Godec, T.; Fang, P.; Ibáñez de Opakua, A.; Henneberg, F.; Godec, A.; Pan, K. T.; Cima-Omori, M.-S.; Chari, A.; Mandelkow, E.; Urlaub, H. et al.; Zweckstetter, M.: Proteasomal degradation of the intrinsically disordered protein tau at single-residue resolution. Science Advances 6 (30), eaba3916 (2020)

Singh, K.; Graf, B.; Linden, A.; Sautner, V.; Urlaub, H.; Tittmann, K.; Stark, H.; Chari, A.: Discovery of a regulatory subunit of the yeast fatty acid synthase. Cell 180 (6), pp. 1130 - 1143.e20 (2020)

Prajapati, S.; Haselbach, D.; Wittig, S.; Patel, M. S.; Chari, A.; Schmidt, C.; Stark, H.; Tittmann, K.: Structural and functional analyses of the human PDH complex suggest a "Division-of-Labor" mechanism by local E1 and E3 clusters. Structure 27 (7), pp. 1124 - 1136 (2019)

Renaud, J. P.; Chari, A.; Ciferri, C.; Liu, W.; Rémigy, H. W.; Stark, H.; Wiesmann, C.: Cryo-EM in drug discovery: Achievements, limitations and prospects. Nature Reviews Drug Discovery 17 (7), pp. 471 - 492 (2018)

Haselbach, D.; Schrader, J.; Lambrecht, F.; Henneberg, F.; Chari, A.; Stark, H.: Long-range allosteric regulation of the human 26S proteasome by 20S proteasome-targeting cancer drugs. Nature Communications 8, 15578 (2017)

Rauhut, R.; Fabrizio, P.; Dybkov, O.; Hartmuth, K.; Pena, V.; Chari, A.; Kumar, V.; Lee, C. T.; Urlaub, H.; Kastner, B. et al.; Stark, H.; Lührmann, R.: Molecular architecture of the Saccharomyces cerevisiae activated spliceosome. Science 353 (6306), pp. 1399 - 1405 (2016)

Paknia, E.; Chari, A.; Stark, H.; Fischer, U.: The ribosome cooperates with the assembly chaperone pICln to initiate formation of snRNPs. Cell Reports 16 (12), pp. 3103 - 3112 (2016)

Schrader, J.; Henneberg, F.; Mata, R. A.; Tittmann, K.; Schneider, T. R.; Stark, H.; Bourenkov, G.; Chari, A.: The inhibition mechanism of human 20S proteasomes enables next-generation inhibitor design. Science 353 (6299), pp. 594 - 598 (2016)