Instruments and Software

Pelco EasyGlow

Plasma cleaner, Glow discharger

Cleaning and charging of cryo-EM grids.

Leica ACE600

Carbon evaporator

Preparation of thin (~2 nm) continuous carbon films for grid preparation.

Thero Fischer Vitrobot (2x)

Plunge freezer

Vitrification of cryo-EM samples on grids. Can be operated in two-side and single-side as well as manual blotting mode. Allows vitrification using ethane and ethane/propane mix.

Zeiss LSM900-cryo

Widefield and confocal laser scanning fluorescence microscopy, Linkam cryo-stage

Fluorescence imaging of vitrified samples on cryo-EM grids (clipped or unclipped). Can be used as upright room-temperature instrument as well.

Thermo Fischer Aquilos 2

Scanning electron microscope with focussed ion beam (FIB-SEM), operates at cryo-temperatures

Preparation of thin lamella from vitrified samples (FIB-milling), mid-end room temperature SEM imaging.

Thermo Fischer Glacios TEM

200 keV field emission gun, 2-lens condenser, single-axis tilt sample holder, Falcon 3EC detector, Autoloader, SerialEM and EPU software

Sample screening and initial data collection.

Thermo Fischer Titan Krios G2

300 keV field emission gun, 3-lens condenser, dual-axis tilt sample holder, Autoloader, Quantum LS energy filter, K3 direct electron detector (post GIF), SerialEM

High-end data acquisition for single particle cryo-EM (AFIS, tilted collection) and cryo-ET (single-site tomography, PACE tomography).