Selected Publications
Proteome analysis of myelin
Please refer to our resource page Myelin Proteome Analysis.
Proteome analysis of brain cells and synapses
Dueking, T.; Spieth, L.; Berghoff, S.A.; Piepkorn, L.; Schmidke, A.M.; Mitkovski, M.; Kannaiyan, N.; Hosang, L.; Scholz, P.; Shaib, A.H.; Schneider, L.V.; Hesse, D.; Ruhwedel, T.; Sun, T.; Linhoff, L.; Trevisiol, A.; Köhler, S.; Marti Pastor, A.; Misgeld, T.; Sereda, M.; Hassouna, I.; Rossner, M.J.; Odoardi, F.; Ischebeck, T.; de Hoz, L.; Hirrlinger, J.; Jahn, O.; Saher, G. Ketogenic diet uncovers differential metabolic plasticity of brain cells. Sci Adv 2022; 8:eabo7639.
Ambrozkiewicz, M.C.; Borisova, E.; Schwark, M.; Ripamonti, S.; Schaub, T.; Smorodchenko, A.; Weber, A.I.; Rhee, H.J.; Altas, B.; Yilmaz, R.; Mueller, S.; Piepkorn, L.; Horan, S.T.; Straussberg, R.; Zaqout, S.; Jahn, O.; Dere, E.; Rosário, M.; Boehm-Sturm, P.; Borck, G.; Willig, K.I.; Rhee, JS.; Tarabykin, V.; Kawabe, H. The murine ortholog of Kaufman oculocerebrofacial syndrome protein Ube3b regulates synapse number by ubiquitinating Ppp3cc. Mol Psychiatry 2021; 26:1980-1995.
Ambrozkiewicz, M.C.; Schwark, M.; Kishimoto-Suga, M.; Borisova, E.; Hori, K.; Salazar-Lázaro, A.; Rusanova, A.; Altas, B.; Piepkorn, L.; Bessa, P.; Schaub, T.; Zhang, X.; Rabe, T.; Ripamonti, S.; Rosário, M.; Akiyama, H.; Jahn, O.; Kobayashi, T.; Hoshino, M.; Tarabykin, V.; Kawabe, H. Polarity acquisition in cortical neurons is driven by synergistic action of Sox9-regulated Wwp1 and Wwp2 E3 ubiquitin ligases and intronic miR-140. Neuron 2018; 100:1097-1115.
Biesemann, C.; Grønborg, M.; Luquet, E.; Wichert, S.P.; Bernard, V.; Bungers, S.R.; Cooper, B.; Varoqueaux, F.; Li, L.; Byrne, J.A.; Urlaub, H.; Jahn, O.; Brose, N.; Herzog, E. Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting. EMBO J 2014; 33:157-170.
Kawabe, H.; Neeb, A.; Dimova, K.; Young, S.M.; Takeda, M.; Katsurabayashi, S.; Mitkovski, M.; Malakhova, O.A.; Zhang, D.-E.; Umikawa, M.; Kariya, K.; Goebbels, S.; Nave, K.-A.; Rosenmund, C.; Jahn, O.; Rhee, J.-S.; Brose, N. Regulation of Rap2A by the ubiquitin ligase Nedd4-1 controls neurite development. Neuron 2010; 65:358-372.
Proteome analysis of the optic nerve
Meschkat, M.; Steyer, A.M.; Weil, M.T.; Kusch, K.; Jahn, O.; Piepkorn, L.; Agüi Gonzalez, P.; Ngoc Phan, N.T.; Ruhwedel, T.; Sadowski, B.; Rizzoli, S.O.; Werner, H.B.; Ehrenreich, H.; Nave, K.-A.; Möbius, W. White matter integrity requires continuous myelin synthesis at the inner tongue. Nat Commun 2022; 13:1163.
Characterization of beta amyloid variants
Klafki, H.W.; Wirths, O.; Mollenhauer, B.; Liepold, T.; Rieper, P.; Esselmann, H.; Vogelgsang, J.; Wiltfang, J.; Jahn, O. Detection and quantification of Aβ−3–40 (APP669-711) in cerebrospinal fluid. J Neurochem 2022; 160:578-589.
Hornung, K.; Zampar, S.; Engel, N.; Klafki, H.; Liepold, T.; Bayer, T.A.; Wiltfang, J.; Jahn, O.; Wirths, O. N-terminal truncated Aβ4-42 is a substrate for neprilysin degradation in vitro and in vivo. J Alzheimers Dis 2019; 67:849-858.
Beyer, I.; Rezaei-Ghaleh, N.; Klafki, H.-W.; Jahn, O.; Haußmann, U.; Wiltfang, J.; Zweckstetter, M.; Knölker, H.-J. Solid-phase synthesis and characterization of N-terminally elongated Aβ−3–x-peptides. Chemistry 2016; 22:8685-8693.
Haußmann, U.; Jahn, O.; Linning, P.; Janßen, C.; Liepold, T.; Portelius, E.; Zetterberg, H.; Bauer, C.; Schuchhardt, J.; Knölker, H.-J.; Klafki, H.; Wiltfang, J. Analysis of amino-terminal variants of amyloid-β peptides by capillary isoelectric focussing immunoassay. Anal Chem 2013; 85:8142-8149.
Schieb, H.; Kratzin, H.; Jahn, O.; Moebius, W.; Rabe, S.; Staufenbiel, M.; Wiltfang, J.; Klafki, H.W. β-Amyloid peptide variants in brains and cerebrospinal fluid from APP transgenic mice: Comparison with human Alzheimer's amyloid. J Biol Chem 2011; 286:33747-33758.
Method development
Kusch, K.; Uecker, M.; Liepold, T.; Möbius, W.; Hoffmann, C.; Neumann, H.; Werner, H.B.; Jahn, O. Partial immunoblotting of 2D-gels: A novel method to identify post-translationally modified proteins exemplified for the myelin acetylome. Proteomes 2017; 5:3.
Kuharev, J.; Navarro, P.; Distler, U.; Jahn, O.; Tenzer, S. In-depth evaluation of software tools for data-independent acquisition-based label-free quantification. Proteomics 2015; 15:3140-3151.
Pettelkau, J.; Ihling, C.H.; Frohberg, P.; van Werven, L.; *Jahn, O.; Sinz, A. Reliable identification of cross-linked products in protein interaction studies by 13C-labeled p-benzoylphenylalanine. J Am Soc Mass Spectrom 2014; 25:1628-1641 (*co-corresponding author).
Schmidt, C.; Hesse, D.; Raabe, M.; Urlaub, H.; Jahn, O. An automated in-gel digestion / iTRAQ-labeling workflow for robust quantification of gel-separated proteins. Proteomics 2013; 13:1417-1422.
Jahn, O.; Hesse, D.; Reinelt, M.; Kratzin, H.D. Technical innovations for the automated identification of gel-separated proteins by MALDI-TOF mass spectrometry. Anal Bioanal Chem 2006; 386:92-103.
Synaptic calmodulin targets & photo affinity labeling
Lipstein, N.; Göth, M.; Piotrowski, C.; Pagel, K.; Sinz, A.; Jahn, O. Presynaptic Calmodulin targets: lessons from structural proteomics. Expert Rev Proteomics 2017; 14:223-242.
Lipstein, N., Schaks, S., Dimova, K., Kalkhof, S., Ihling, C., Kölbel, K., Ashery, U., Rhee, J., Brose, N., Sinz, A., Jahn, O. Nonconserved Ca2+/calmodulin binding sites in Munc13s differentially control synaptic short-term plasticity. Mol Cell Biol 2012; 32:4628-4641.
Rodriguez-Castaneda, F.; Maestre-Martinez, M.; Coudevylle, N.; Dimova, K.; Junge, H.; Lipstein, N.; Lee, D.; Becker, S.; Brose, N.; Jahn, O.; Carlomagno, T.; Griesinger, C. Modular architecture of Munc13/calmodulin complexes: Dual regulation by Ca2+ and possible function in short-term synaptic plasticity. EMBO J 2010; 29:680-691.
Dimova, K.; Kalkhof, S.; Pottratz, I.; Ihling, C.; Rodriguez-Castaneda, F.; Liepold, T.; Griesinger, C.; Brose, N.; Sinz, A.; Jahn, O. Structural insights into the calmodulin - Munc13 interaction obtained by cross-linking and mass spectrometry. Biochemistry 2009; 48:5908-5921.
Dimova, K.; Kawabe, H.; Betz, A.; Brose, N.; Jahn, O. Characterization of the Munc13-calmodulin interaction by photoaffinity labeling. Biochim Biophys Acta 2006; 1763:1256-1265.