Selected Publications

Biomarker for neuropsychiatric diseases

Please refer to Translational Neuroproteomics.

Myelin

Please refer to our Myelin Proteome Resource.

Cells and synapses of the brain

Altas, B.; Tuffy, L.P.; Patrizi, A.; Dimova, K.; Soykan, T.; Brandenburg, C.; Romanowski, A.J.; Whitten, J.R.; Robertson, C.D.; Khim, S.N.; Crutcher G.W.; Ambrozkiewicz, M.C.; Yagensky, O.; Krueger-Burg, D.; Hammer, M.; Hsiao, H.H.; Laskowski, P.R.; Dyck, L.; Puche, A.C.; Sassoè-Pognetto, M.; Chua, J.J.E.; Urlaub, H.; Jahn, O.; Brose, N.; Poulopoulos, A. Region-specific phosphorylation determines Neuroligin-3 localization to excitatory versus inhibitory synapses. Biol Psychiatry 2024; 96:815-828.

Altas, B.; Rhee, H.-J.; Ju, A.; Cruces Solís, H.; Karaca, S.; Winchenbach, J.; Kaplan-Arabaci, Ö.; Schwark, M.; Ambrozkiewicz, M.C.; Lee, C.; Spieth, L.; Wieser, G.L.; Chaugule, V.K.; Majoul, I.; Hassan, M.A.; Goel, R.; Wojcik, S.M.; Koganezawa, N.; Hanamura, K.; Rotin, D.; Pichler, A.; Mitkovski, M.; de Hoz, L.; Poulopoulos, A.; Urlaub, H.; Jahn, O.; Saher, G.; Brose, N.; Rhee, J.; Kawabe, H. Nedd4-2-dependent regulation of astrocytic Kir4.1 and Connexin43 controls neuronal network activity. J Cell Biol 2024; 223:e201902050.

Dueking, T.; Spieth, L.; Berghoff, S.A.; Piepkorn, L.; Schmidke, A.M.; Mitkovski, M.; Kannaiyan, N.; Hosang, L.; Scholz, P.; Shaib, A.H.; Schneider, L.V.; Hesse, D.; Ruhwedel, T.; Sun, T.; Linhoff, L.; Trevisiol, A.; Köhler, S.; Marti Pastor, A.; Misgeld, T.; Sereda, M.; Hassouna, I.; Rossner, M.J.; Odoardi, F.; Ischebeck, T.; de Hoz, L.; Hirrlinger, J.; Jahn, O.; Saher, G. Ketogenic diet uncovers differential metabolic plasticity of brain cells. Sci Adv 2022; 8:eabo7639.

Ambrozkiewicz, M.C.; Borisova, E.; Schwark, M.; Ripamonti, S.; Schaub, T.; Smorodchenko, A.; Weber, A.I.; Rhee, H.J.; Altas, B.; Yilmaz, R.; Mueller, S.; Piepkorn, L.; Horan, S.T.; Straussberg, R.; Zaqout, S.; Jahn, O.; Dere, E.; Rosário, M.; Boehm-Sturm, P.; Borck, G.; Willig, K.I.; Rhee, JS.; Tarabykin, V.; Kawabe, H. The murine ortholog of Kaufman oculocerebrofacial syndrome protein Ube3b regulates synapse number by ubiquitinating Ppp3cc. Mol Psychiatry 2021; 26:1980-1995.

Ambrozkiewicz, M.C.; Schwark, M.; Kishimoto-Suga, M.; Borisova, E.; Hori, K.; Salazar-Lázaro, A.; Rusanova, A.; Altas, B.; Piepkorn, L.; Bessa, P.; Schaub, T.; Zhang, X.; Rabe, T.; Ripamonti, S.; Rosário, M.; Akiyama, H.; Jahn, O.; Kobayashi, T.; Hoshino, M.; Tarabykin, V.; Kawabe, H. Polarity acquisition in cortical neurons is driven by synergistic action of Sox9-regulated Wwp1 and Wwp2 E3 ubiquitin ligases and intronic miR-140. Neuron 2018; 100:1097-1115.

Optic nerve and retina

Asadollahi, E.; Trevisiol, A.; Saab, A.S.; Looser, Z.J.; Dibaj, P.; Ebrahimi, R.; Kusch, K.; Ruhwedel, T.; Möbius, W.; Jahn, O.; Lee, J.Y.; Don, A.S.; Khalil, M.-A.; Hiller, K.; Baes, M.; Weber, B.; Abel, E.D.; Balabio, A.; Popko, B.; Kassmann, C.M.; Ehrenreich, H.; Hirrlinger, J.; Nave, K.-A. Oligodendroglial fatty acid metabolism as a central nervous system energy reserve. Nat Neurosci 2024; 27:1934-1944.

Lux, U.T.; Meyer, J.; Jahn, O.; Davison, A.; Babai, N.; Gießl, A.; Wartenberg, A.; Sticht, H.; Brose, N.; Reim, K.; Brandstätter, J.H. Light-dependent regulation of neurotransmitter release from rod photoreceptor ribbon synapses involves an interplay of Complexin 4 and Transducin with the SNARE complex. Front Mol Neurosci; 17:1308466.

Meschkat, M.; Steyer, A.M.; Weil, M.T.; Kusch, K.; Jahn, O.; Piepkorn, L.; Agüi Gonzalez, P.; Ngoc Phan, N.T.; Ruhwedel, T.; Sadowski, B.; Rizzoli, S.O.; Werner, H.B.; Ehrenreich, H.; Nave, K.-A.; Möbius, W. White matter integrity requires continuous myelin synthesis at the inner tongue. Nat Commun 2022; 13:1163.

Tools and Resources

Lohse, M.; Sun, S.; Fiosins, M.; Bonn, S.; Zamorano, P.; Jahn, O.; Lipstein, N. Investigating the molecular composition of neuronal subcompartments using proximity labeling. Methods Mol Biol 2025; 2910:105-125. In: Soykan, T. and Poulopoulos, A. (eds); Synapse Development, 2^nd edition. Humana Press, New York, NY.

Liepold, T.; Klafki, H.W.; Kumar, S.; Walter, J.; Wirths, O.; Wiltfang, J.; Jahn, O. Matrix development for the detection of phosphorylated Amyloid-β peptides by MALDI-TOF-MS. J Am Soc Mass Spectrom 2023; 34:505-512.

Kuharev, J.; Navarro, P.; Distler, U.; Jahn, O.; Tenzer, S. In-depth evaluation of software tools for data-independent acquisition-based label-free quantification. Proteomics 2015; 15:3140-3151.

Schmidt, C.; Hesse, D.; Raabe, M.; Urlaub, H.; Jahn, O. An automated in-gel digestion / iTRAQ-labeling workflow for robust quantification of gel-separated proteins. Proteomics 2013; 13:1417-1422.

Jahn, O.; Hesse, D.; Reinelt, M.; Kratzin, H.D. Technical innovations for the automated identification of gel-separated proteins by MALDI-TOF mass spectrometry. Anal Bioanal Chem 2006; 386:92-103.

Synaptic calmodulin targets & photo affinity labeling

Lipstein, N.; Göth, M.; Piotrowski, C.; Pagel, K.; Sinz, A.; Jahn, O. Presynaptic Calmodulin targets: lessons from structural proteomics. Expert Rev Proteomics 2017; 14:223-242.

Pettelkau, J.; Ihling, C.H.; Frohberg, P.; van Werven, L.; *Jahn, O.; Sinz, A. Reliable identification of cross-linked products in protein interaction studies by 13C-labeled p-benzoylphenylalanine. J Am Soc Mass Spectrom 2014; 25:1628-1641 (*co-corresponding author).

Lipstein, N., Schaks, S., Dimova, K., Kalkhof, S., Ihling, C., Kölbel, K., Ashery, U., Rhee, J., Brose, N., Sinz, A., Jahn, O. Nonconserved Ca2+/calmodulin binding sites in Munc13s differentially control synaptic short-term plasticity. Mol Cell Biol 2012; 32:4628-4641.

Rodriguez-Castaneda, F.; Maestre-Martinez, M.; Coudevylle, N.; Dimova, K.; Junge, H.; Lipstein, N.; Lee, D.; Becker, S.; Brose, N.; Jahn, O.; Carlomagno, T.; Griesinger, C. Modular architecture of Munc13/calmodulin complexes: Dual regulation by Ca2+ and possible function in short-term synaptic plasticity. EMBO J 2010; 29:680-691.

Dimova, K.; Kalkhof, S.; Pottratz, I.; Ihling, C.; Rodriguez-Castaneda, F.; Liepold, T.; Griesinger, C.; Brose, N.; Sinz, A.; Jahn, O. Structural insights into the calmodulin - Munc13 interaction obtained by cross-linking and mass spectrometry. Biochemistry 2009; 48:5908-5921.

Dimova, K.; Kawabe, H.; Betz, A.; Brose, N.; Jahn, O. Characterization of the Munc13-calmodulin interaction by photoaffinity labeling. Biochim Biophys Acta 2006; 1763:1256-1265.