Since 2018, the Live-cell Imaging facility provides access to state-of-the art microscopy and biophysical technologies, as well as comprehensive support from sample preparation to assay development and image data analysis - making these services available to all researchers at the Institute. The facility assists users in sample preparation by providing reagents and helps develop experimental assays to take advantage of the newest microscopy technologies to image live cells in culture as well as intact embryos, tissues, and organoids. We also provide support in microscope automation (adaptive-feedback/smart microscopy) as well as image and data analysis.
Current equipment includes several high-end confocal systems (Zeiss LSM780, Zeiss LSM880AiryFast and Visitron Systems Spinning-Disk), a STED super-resolution microscope (Aberrior Instruments STEDYCON), a light sheet microscope (Zeiss LSZ1), two FACS machines (SONY SH800 and BD ARIA II), a plate reader (Biotek), and a mass photometer (Refeyn).
The facility also provides access to rooms for cell culture, microinjection, and sample preparation. For image processing and data analysis the facility has several remotely accessible Virtual Machines (VMs) running on state-of-the-art high performance CPU/GPU servers integrated with >100 TB of storage. Through the VM the facility provides access to a limited number of licenses for Imaris and Arivis (4D rendering and analysis), Huygens (deconvolution and localization), and open-source software.