Publications

52.

Journal Article

Schneider, C.; Agafonov, D. E.; Schmitzova, J.; Hartmuth, K.; Fabrizio, P.; Lührmann, R.: Dynamic contacts of U2, RES, Cwc25, Prp8 and Prp45 proteins with the Pre-mRNA branch-site and 3' splice site during catalytic activation and step 1 catalysis in yeast spliceosomes. PLoS Genetics 11 (9), e1005539 (2015)

MPG.PuRe

53.

Journal Article

Boesler, C.; Rigo, N.; Agafonov, D. E.; Kastner, B.; Urlaub, H.; Will, C. L.; Lührmann, R.: Stable tri-snRNP integration is accompanied by a major structural rearrangement of the spliceosome that is dependent on Prp8 interaction with the 5‘ splice site. RNA 21 (11), pp. 1993 - 2005 (2015)

MPG.PuRe

54.

Journal Article

Benito, E.; Urbanke, H.; Ramachandran, B.; Barth, J.; Halder, R.; Awasthi, A.; Jain, G.; Capece, J. G.; Burkhardt, S.; Navarro-Sala, M. et al.; Nagarajan, S.; Schütz, A. L.; Johnsen, S. A.; Bonn, S.; Lührmann, R.; Dean, C.; Fischer, A.: HDAC inhibitor-dependent transcriptome and memory reinstatement in cognitive decline models. Journal of Clinical Investigation 125 (9), pp. 3572 - 3584 (2015)

MPG.PuRe

55.

Journal Article

Wahl, M. C.; Lührmann, R.: Snapshot: Spliceosome dynamics III. Cell 162 (3), pp. 690 - 690.e1 (2015)

MPG.PuRe

56.

Journal Article

Wahl, M. C.; Lührmann, R.: SnapShot: Spliceosome dynamics II. Cell 162 (2), pp. 456 - 456.e1 (2015)

MPG.PuRe

57.

Journal Article

Liu, S.; Mozaffari-Jovin, S.; Wollenhaupt, J.; Santos, , K. F.; Theuser, M.; Dunin-Horkawicz, S.; Fabrizio, P.; Bujnicki, J. M.; Lührmann, R.; Wahl, M. C.: A composite double-/single-stranded RNA-binding region in protein Prp3 supports tri-snRNP stability and splicing. eLife 4, e07320 (2015)

MPG.PuRe

58.

Journal Article

Wahl, M. C.; Lührmann, R.: SnapShot: Spliceosome dynamics I. Cell 161 (6), pp. 1474 - 1474.e1 (2015)

MPG.PuRe

59.

Journal Article

De, I.; Bessonov, S.; Hofele, R.; Dos Santos, K.; Will, C. L.; Urlaub, H.; Lührmann, R.; Pena, V.: The RNA helicase Aquarius exhibits structural adaptations mediating its recruitment to spliceosomes. Nature Structural and Molecular Biology 22 (2), pp. 138 - 144 (2015)

MPG.PuRe

60.

Journal Article

Warkocki, Z.; Schneider, C.; Mozaffari-Jovin, S.; Schmitzova, J.; Höbartner, C.; Fabrizio, P.; Lührmann, R.: The G-patch protein Spp2 couples the spliceosome-stimulated ATPase activity of the DEAH-box protein Prp2 to catalytic activation of the spliceosome. Genes and Development 29 (1), pp. 94 - 107 (2015)

MPG.PuRe

61.

Journal Article

Pawellek, A.; McElroy, S.; Samatov, T.; Mitchell, L.; Woodland, A.; Ryder, U.; Gray, D.; Lührmann, R.; Lamond, A.: Identification of small molecule inhibitors of pre-mRNA splicing. The Journal of Biological Chemistry 289 (50), pp. 34683 - 34698 (2014)

MPG.PuRe

62.

Journal Article

Alló, M.; Agirre, E.; Bessonov, S.; Bertucci, P.; Acuña, L. G.; Buggiano, V.; Bellora, N.; Singh, B.; Petrilloa, E.; Blaustein, M. et al.; Miñana, B.; Dujardin, G.; Pozzi, B.; Pelisch, F.; Bechara, E.; Agafonov, D. E.; Srebrow, A.; Lührmann, R.; Valcárcel, J.; Eyras, E.; Kornblihtt, A. R.: Argonaute-1 binds transcriptional enhancers and controls constitutive and alternative splicing in human cells. Proceedings of the National Academy of Sciences of the United States of America 111 (44), pp. 15622 - 15629 (2014)

MPG.PuRe

63.

Journal Article

Kramer, K.; Sachsenberg, T.; Beckmann, B. M.; Qamar, S.; Boon, K. L.; Hentze, M. W.; Kohlbacher, O.; Urlaub, H.: Photo-cross-linking and high-resolution mass spectrometry for assignment of RNA-binding sites in RNA-binding proteins. Nature Methods 11 (10), pp. 1064 - 1070 (2014)

MPG.PuRe

64.

Journal Article

Wysoczanski, P.; Schneider, C.; Xiang, S. Q.; Munari, F.; Trowitzsch, S.; Wahl, M. C.; Lührmann, R.; Becker, S.; Zweckstetter, M.: Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex. Nature Structural and Molecular Biology 21 (10), pp. 911 - 918 (2014)

MPG.PuRe

65.

Journal Article

Möhlmann, S.; Mathew, R.; Neumann, P.; Schmitt, A.; Lührmann, R.; Ficner, R.: Structural and functional analysis of the human spliceosomal DEAD-box helicase Prp28. Acta Crystallographica D 70 (6), pp. 1622 - 1630 (2014)

MPG.PuRe

66.

Journal Article

Mozaffari-Jovin, S.; Wandersleben, T.; Santos, K. F.; Will, C. L.; Lührmann, R.; Wahl, M. C.: Novel regulatory principles of the spliceosomal Brr2 RNA helicase and links to retinal disease in humans. RNA Biology 11 (4), pp. 298 - 312 (2014)

MPG.PuRe

67.

Journal Article

Schmidt, C.; Groenborg, M.; Deckert, J.; Bessonov, S.; Conrad, T.; Lührmann, R.; Urlaub, H.: Mass spectrometry–based relative quantification of proteins in precatalytic and catalytically active spliceosomes by metabolic labeling (SILAC), chemical labeling (iTRAQ), and label-free spectral count. RNA 20 (3), pp. 406 - 420 (2014)

MPG.PuRe

68.

Journal Article

Xiang, S. Q.; Gapsys, V.; Kim, H. Y.; Bessonov, S.; Hsiao, H. H.; Möhlmann, S.; Klaukien, V.; Ficner, R.; Becker, S.; Urlaub, H. et al.; Lührmann, R.; de Groot, B.; Zweckstetter, M.: Phosphorylation drives a dynamic switch in serine/arginine-rich proteins. Structure 21 (12), pp. 2162 - 2174 (2013)

MPG.PuRe

69.

Journal Article

Anokhina, M.; Bessonov, S.; Miao, Z. C.; Westhof, E.; Hartmuth, K.; Lührmann, R.: RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic core. EMBO Journal 32 (21), pp. 2804 - 2818 (2013)

MPG.PuRe

70.

Journal Article

Hofmann, J. C.; Tegha-Dunghu, J.; Dräger, S.; Will, C. L.; Lührmann, R.; Gruss, O. J.: The Prp19 complex directly functions in mitotic spindle assembly. PLoS One 8 (9), e74851 (2013)

MPG.PuRe

71.

Journal Article

Mozaffari-Jovin, S.; Wandersleben, T.; Santos, K. F.; Will, C. L.; Lührmann, R.; Wahl, M. C.: Inhibition of RNA helicase Brr2 by the C-terminal tail of the spliceosomal protein Prp8. Science 341 (6141), pp. 80 - 84 (2013)

MPG.PuRe

72.

Journal Article

Ohrt, T.; Odenwälder, P.; Dannenberg, J.; Prior , M.; Warkocki, Z.; Schmitzova, J.; Karaduman, R.; Gregor, I.; Enderlein, J.; Fabrizio, P. et al.; Lührmann, R.: Molecular dissection of step 2 catalysis of yeast pre-mRNA splicing investigated in a purified system. RNA 19 (7), pp. 902 - 915 (2013)

MPG.PuRe

73.

Journal Article

Weber, G.; Cristao, V. F.; Santos, K. F.; Mozaffari-Jovin, S.; Heroven, A. C.; Holton, N.; Lührmann, R.; Beggs, J. D.; Wahl, M. C.: Structural basis for dual roles of Aar2p in U5 snRNP assembly. Genes and Development 27 (5), pp. 525 - 540 (2013)

MPG.PuRe

74.

Journal Article

Fourmann, J. B.; Schmitzova, J.; Christian, H.; Urlaub, H.; Ficner, R.; Boon, K. L.; Fabrizio, P.; Lührmann, R.: Dissection of the factor requirements for spliceosome disassembly and the elucidation of its dissociation products using a purified splicing system. Genes and Development 27 (4), pp. 413 - 428 (2013)

MPG.PuRe

75.

Journal Article

Mozaffari-Jovin, S.; Santos, K. F.; Hsiao, H. H.; Will, C. L.; Urlaub, H.; Wahl, M. C.; Lührmann, R.: The Prp8 RNase H-like domain inhibits Brr2-mediated U4/U6 snRNA unwinding by blocking Brr2 loading onto the U4 snRNA. Genes and Development 26 (23), pp. 2422 - 2434 (2012)

MPG.PuRe

76.

Journal Article

Schmitzova, J.; Pena, V.: Emerging views about the molecular structure of the spliceosomal catalytic center. RNA Biology 9 (11), pp. 1311 - 1318 (2012)

MPG.PuRe

77.

Journal Article

Santos , K. F.; Mozaffari-Jovin, S.; Weber , G.; Pena, V.; Lührmann, R.; Wahl, M. C.: Structural basis for functional cooperation between tandem helicase cassettes in Brr2-mediated remodeling of the spliceosome. Proceedings of the National Academy of Sciences of the United States of America 109 (43), pp. 17418 - 17423 (2012)

MPG.PuRe

78.

Journal Article

Patil, V.; Canzoneri, J. C.; Samatov, T. R.; Lührmann, R.; Oyelere, A. K.: Molecular architecture of Zinc chelating small molecules that inhibit spliceosome assembly at an early stage. RNA 18 (9), pp. 1605 - 1611 (2012)

MPG.PuRe

79.

Journal Article

Simoes-Barbosa, A.; Chakrabarti, K.; Pearson, M. D.; Benarroch, D.; Shuman, S.; Johnson, P. J.: Box H/ACA snoRNAs are preferred substrates for the trimethylguanosine synthase in the divergent unicellular eukaryote Trichomonas vaginalis. RNA 18 (9), pp. 1656 - 1665 (2012)

MPG.PuRe

80.

Journal Article

Girard, C.; Will, C. L.; Peng, J.; Makarov, E. M.; Kastner, B.; Lemm, I.; Urlaub, H.; Hartmuth, K.; Lührmann, R.: Retention of posttranscriptional spliceosomes in nuclear speckles until splicing completion. Nature communications 3, 994 (2012)

MPG.PuRe

81.

Journal Article

Wolf, E.; Kastner, B.; Lührmann, R.: Antisense-targeted immuno-EM localization of the pre-mRNA path in the spliceosomal C complex. RNA 18 (7), pp. 1347 - 1357 (2012)

MPG.PuRe

82.

Journal Article

Rasche, N.; Dybkov, O.; Schmitzova, J.; Akyildiz, B.; Fabrizio, P.; Lührmann, R.: Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre. EMBO Journal 31 (6), pp. 1591 - 1604 (2012)

MPG.PuRe

83.

Journal Article

Samatov, T. R.; Wolf, A.; Odenwälder, P.; Bessonov, S.; Deraeve, C.; Bon, R. S.; Waldmann, H.; Lührmann, R.: Psoromic acid derivatives: A new family of small-molecule pre-mRNA splicing inhibitors discovered by a stage-specific high-throughput in vitro splicing assay. ChemBioChem 13 (5), pp. 640 - 644 (2012)

MPG.PuRe

84.

Journal Article

Hegele, A.; Kamburov, A.; Grossmann, A.; Sourlis, C.; Wowro, S.; Weimann, M.; Will, C. L.; Pena, V.; Lührmann, R.; Stelzl, U.: Dynamic protein-protein interaction wiring of the human spliceosome. Molecular Cell 45 (4), pp. 567 - 580 (2012)

MPG.PuRe

85.

Journal Article

Ohrt, T.; Prior, M.; Dannenberg, J.; Odenwälder, P.; Dybkov, O.; Rasche, N.; Schmitzova, J.; Gregor, I.; Fabrizio, P.; Enderlein, J. et al.; Lührmann, R.: Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS. RNA 18 (6), pp. 1244 - 1256 (2012)

MPG.PuRe

86.

Journal Article

Schmitzova, J.; Rasche, N.; Dybkov, O.; Kramer, K.; Fabrizio, P.; Urlaub, H.; Lührmann, R.; Pena, V.: Crystal structure of Cwc2 reveals a novel architecture of a multipartite RNA-binding protein. EMBO Journal 31 (9), pp. 2222 - 2234 (2012)

MPG.PuRe

87.

Journal Article

Liu, S.; Ghalei, H.; Lührmann, R.; Wahl, M.: Structural basis for the dual U4 and U4atac snRNA-binding specificity of spliceosomal protein hPrp31. RNA 17 (9), pp. 1655 - 1663 (2011)

MPG.PuRe

88.

Journal Article

Agafonov, D. E.; Deckert, J.; Wolf, E.; Odenwälder, P.; Bessonov, S.; Will, C. L.; Urlaub, H.; Lührmann, R.: Semiquantitative proteomic analysis of the human spliceosome via a novel two-dimensional gel electrophoresis method. Molecular and Cellular Biology 31 (13), pp. 2667 - 2682 (2011)

MPG.PuRe

89.

Journal Article

Zhang, Z.; Kelemen, O.; van Santen, M. A.; Yelton, S. M.; Wendlandt, A. E.; Sviripa, V. M.; Bollen, M.; Beullens, M.; Urlaub, H.; Lührmann, R. et al.; Watt, D. S.; Stamm, S.: Synthesis and characterization of pseudocantharidins, novel phosphatase modulators that promote the inclusion of exon 7 into the SMN (survival of motoneuron) pre-mRNA. Journal of Biological Chemistry 286 (12), pp. 10126 - 10136 (2011)

MPG.PuRe

90.

Journal Article

Popow, J.; Englert, M.; Weitzer, S.; Schleiffer, A.; Mierzwa, B.; Mechtler, K.; Trowitzsch, S.; Will, C. L.; Lührmann, R.; Soell, D. et al.; Martinez, J.: HSPC117 is the essential subunit of a human tRNA splicing ligase complex. Science 331 (6018), pp. 760 - 764 (2011)

MPG.PuRe

91.

Journal Article

Golas, M. M.; Sander, B.; Bessonov, S.; Grote, M.; Wolf, E.; Kastner, B.; Stark, H.; Lührmann, R.: 3D cryo-EM structure of an active step I spliceosome and localization of its catalytic core. Molecular Cell 40 (6), pp. 927 - 938 (2010)

MPG.PuRe

92.

Journal Article

Bessonov, S.; Anokhina, M.; Krasauskas, A.; Golas, M. M.; Sander, B.; Will, C. L.; Urlaub, H.; Stark, H.; Lührmann, R.: Characterization of purified human Bact spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysis. RNA 16 (12), pp. 2384 - 2403 (2010)

MPG.PuRe

93.

Journal Article

Sander, B.; Golas, M. M.; Lührmann, R.; Stark, H.: An approach for de novo structure determination of dynamic molecular assemblies by electron cryomicroscopy. Structure 18 (6), pp. 667 - 676 (2010)

MPG.PuRe

94.

Journal Article

Grote, M.; Wolf, E.; Will, C. L.; Lemm, I.; Agafonov, D. E.; Schomburg, A.; Fischle, W.; Urlaub, H.; Lührmann, R.: Molecular architecture of the human Prp19/CDC5L complex. Molecular and Cellular Biology 30 (9), pp. 2105 - 2119 (2010)

MPG.PuRe

95.

Journal Article

Kühn-Hölsken, E.; Lenz, C.; Dickmanns, A.; Hsiao, H. H.; Richter, F. M.; Kastner, B.; Ficner, R.; Urlaub, H.: Mapping the binding site of snurportin 1 on native U1 snRNP by cross-linking and mass spectrometry. Nucleic Acids Research 38 (16), pp. 5581 - 5593 (2010)

MPG.PuRe

96.

Journal Article

Schneider, M.; Will, C. L.; Anokhina, M.; Tazi, J.; Urlaub, H.; Lührmann, R.: Exon definition complexes contain the tri-snRNP and can be directly converted into B-like precatalytic splicing complexes. Molecular Cell 38 (2), pp. 223 - 235 (2010)

MPG.PuRe

97.

Journal Article

Stegmann, C.; Lührmann, R.; Wahl, M. C.: The crystal structure of PPIL1 bound to cyclosporine A suggests a binding mode for a linear epitope of the SKIP protein. PLoS ONE 5, e10013 (2010)

MPG.PuRe

98.

Journal Article

Strzelecka, M.; Trowitzsch, S.; Weber, G.; Lührmann, R.; Oates, A. C.; Neugebauer, K. M.: Coilin-dependent snRNP assembly is essential for zebrafish embryogenesis. Nature Structural and Molecular Biology 17 (4), pp. 403 - 409 (2010)

MPG.PuRe

99.

Journal Article

Verbeeren, J.; Niemelae, E. H.; Turunen, J. J.; Will, C. L.; Ravanti, J. J.; Lührmann, R.; Frilander, M. J.: An ancient mechanism for splicing control: U11 snRNP as an activator of alternative splicing. Molecular Cell 37 (6), pp. 821 - 833 (2010)

MPG.PuRe

100.

Journal Article

Schneider, M.; Hsiao, H. H.; Will, C. L.; Giet, R.; Urlaub, H.; Lührmann, R.: Human PRP4 kinase is required for stable tri-snRNP association during spliceosomal B complex formation. Nature Structural and Molecular Biology 17 (2), pp. 216 - 222 (2010)

MPG.PuRe